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To determine the purity of your sample, use the qubit® dsdna br assay kit together with the qubit® rna br assay kit The kit contains reagents to dilute dna samples into a working solution and dna standards to calibrate the qubit fluorometer. These measurements give you a much better indication of sample purity than that produced by measuring the a260/a280 ratio.
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This equation generates a result with the same units as the value given by the qubit® 2.0 fluorometer (i.e., if the qubit® 2.0 fluorometer gave a concentration in μg/ml, the result of the equation will be in μg/ml). As techniques evolve, especially in synthetic biology, bioengineering, and metagenomics, accurate quantification of dsdna using kits like qubit br will remain a cornerstone protocol. Plug in and turn on qubit 4.0 fluorometer
Calibrate the qubit using the standards
From the initial home screen, select “dna”, then select the assay type “dsdna broad range” Select the “read standards” option Insert the tube std 1 into the chamber Close the lid and select “read standard.”
This protocol assumes that you are preparing standards for calibrating the qubittm fluorometer If you plan to use the last calibration performed on the instrument, fewer tubes (step 1) and less working solution (step 3) will be needed (see “calibrate the qubittm fluorometer” on page 2). To determine the purity of your sample, use the qubit® dsdna br assay kit together with the qubit® rna assay kit These measurements will give you a much better indication of sample purity than that produced by an a260/a280 measurement.
